This was the debut for Wile E. Coyote and the Road Runner. It was also their only cartoon made in the 1940s. It set the template for the series, in which Wile E. Coyote (here given the ersatz Latin name Carnivorous Vulgaris) tries to catch Roadrunner (Accelleratii Incredibus) through many traps, plans and products, although in this first cartoon not all of the products are yet made by the Acme Corporation.
Road Runner (voice)
Coyote Effects (voice)